| dc.description.abstract | Toll-like receptor 4 and myxovirus genes have been demonstrated to confer
resistance to Salmonella and Newcastle disease (ND), respectively. These genes
have not yet been investigated in Sentul chickens. Therefore, the aim of this study
was to determine the polymorphisms and association of the TLR4 and Mx genes
as candidate genes underlying resistance to Salmonella and ND in selected Sentul
chickens. One hundred and eighty-five Sentul chickens were genotyped using
PCR-RFLP. The genotype and allele frequencies, polymorphic information
contents and Hardy-Weinberg equilibrium (HWE) statuses were analysed. The
genotypes were associated with immunoglobulin Y (IgY), Salmonella-specific
antibodies and Newcastle disease-specific antibodies. These parameters were
determined using indirect ELISA, clearance tests and haemagglutination
inhibition, respectively. The immune traits were further grouped into high,
medium and low categories. The data were analysed using the GLM and t-test.
Polymorphisms in TLR4|MscI and Mx|Hpy8I were observed. The
population was in HWE for both genes. The frequencies of the TLR4 allele G and
genotype GG were significantly high (P<0.05) in chickens with high immune
traits. The genotype GG of TLR4 gene recorded significantly higher immune
traits than genotypes AA and AG. For the Mx gene, the frequency of allele A was
higher than of allele G at a high IgY titre. TLR4|MscI and Mx|Hpy8I are potential
markers for selection against Salmonella and Newcastle disease in Sentul
chickens. However, further investigations are recommended. | id |
