dc.contributor.author | Harlina, Eva | |
dc.contributor.author | Priyosuryanto, Bambang Pantjo | |
dc.contributor.author | Riadi, Yudi | |
dc.date.accessioned | 2010-04-29T08:34:30Z | |
dc.date.available | 2010-04-29T08:34:30Z | |
dc.date.issued | 2002 | |
dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/9695 | |
dc.description.abstract | Endothelial cells derived from femoralis artery of rabbit were succesfully cultured in vitro in liquid medium of Dulbeco modified essential medium-Ham’s F-12 (DME/F-12) and semi solid medium of collagen gel. The cells cultured in liquid medium grew and formed colony with a pattern of monolayer sheet. They were round to oval and spindle in shape. The cells were grown in collagen gel by using floating and gel-embedded system to show their three-dimensional structure. The growth pattern of the cells cultured in both collagen gel systems showed a formation of irregular fibrous-like structure, elongated cells and colony with duct-like structure as well as radiating projection pattern and formation of a large lumen like structure. Histological findings using Haematoxylin and Eosin staining showed development of a compact cell colony which formed a monolayer sheet with a cell layer of fibroblast and a thin collagen layer. Immunohistochemically, endothelial cells showed positive reaction for anti-Factor VIII Related-Ag (F VIII R-Ag), with smooth fiber pattern in cytoplasm running along the cell border. | id |
dc.publisher | IPB (Bogor Agricultural University) | |
dc.title | Studi In Vitro Biakan Sel Endotel Kelinci pada Media Cair dan Semi Padat | id |