Cryopreservation of Balinese Bull Semen Using Three Different Types of Extenders and Equilibration Times
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Date
2018Author
Saber Abdel-Rahman, Amal
Arifiantini, Iis
Setiadi, Mohamed
Said, Syahruddin
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Show full item recordAbstract
Bali cattle (bos javanicus) is considered as the most preferable breed in the
small holding systems in Indonesia because of their high resistance against
diseases, their remarkable ability to grow on low quality fodders and their high
quality meat. However there is a decrease in the total population of the Bali cattle
which could be because of the excessive slaughtering such a problem can be
overcome by applying strict management planning.
The quality of the insemination dose plays an important role in the success
of the artificial insemination process however in compare with fresh semen; the
post-thawed semen quality is generally lower because of the cryo-injury which
could lead to the decrease in sperm motility, viability and membrane integrity.
Another problem is regarding the needed equilibration time. Generally four hours
equilibration time is applied before semen freezing which is considered as a time
consuming step, moreover there are a desire for the reduction of such time.
The objectives of the present study were to compare the post-thawed quality
of Balinese bull sperm cryopreserved in three different extenders; Tris-clarified
egg yolk (Tris-cEY), Bioxcell® and Optixcell® extenders in combination with
three different equilibration times (30min, 2hrs and 4hrs).
Thirty six ejaculates were collected from six Balinese bulls and were frozen
in three different extenders (Tris-cEY, Bioxcell®and Optixcell® extenders) after
equilibration in three different equilibration times (30 min, 2hrs and 4hrs) by
following the routine freezing protocol. The post-thawed semen parameters were
evaluated for motility using computer-assisted sperm analysis (CASA), membrane
integrity using hypo-osmotic swelling test (HOST) and for viability using eosin
nigrosin staining.
There were significant interactions between equilibration time and extender
type for sperm motility, viability and membrane integrity. Thirty minutes
equilibration time had the lowest values (P<0.05) for total and progressive
motilities, percentages of sperm with intact plasma membrane and viability
independent of extender type. Sperm velocity parameters and membrane integrity
were better in semen frozen with Optixcell® than commercial lecithin based and
Tris-cEY extenders. Overall, semen extended in Tris-cEY, Optixcell® and
Bioxcell®were better equilibrated at 4 hrs however, commercial Liposome based
extender equilibrated at 2 hrs showed good motility and viability which were not
significantly different from 4hrs equilibration (P>0.05).
In conclusion, based on the evaluations of sperm motility, viability and
functional membrane integrity, thirty minutes and two hrs equilibration were not
sufficient for maintaining the post-thawed semen quality. Semen equilibration at 4
hrs. yielded the greatest sperm survival, independent of the used extender type.
Semen which was equilibrated with Optixcell® extender for 2 hrs showed good
motility and viability however more evaluations regarding the acrosome status
and DNA fragmentation are needed. Either Bioxcell® or Optixcell® could be
used for Balinese bull semen cryopreservation.
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- MT - Veterinary Science [931]