| dc.description.abstract | Germplasm is a source of genetic diversity that is essential for sustained through the long
term. Seeds germplasms storage that are not resistant to desiccation can be carried out by
cryopreservation. Cryopreservation is a method of storing the plant material in liquid nitrogen
( -196oC) so the metabolic processes in cells, tissues or organs that are stored can be stopped
so the plant material can be stored in a very long time without a genetic change or somaclonal
variation. Cryoprotectants in cryopreservation storage is needed for plant material protection
to adapt to the very low temperature. Some examples of successful treated-plant for
cryopreservation are cocoa and papaya. In cocoa, proembryo somatic derived from
cryopreservation cocoa zygotic embryo can creating embryos survived and were able to grow
a callus back as much as 46.67% is dimethylsulfoxide (DMSO) and glycerol with a
concentration of 15% each in MSO solution, at a temperature of thawing (melting) 35oC by
using culture media such as media restorer MS + picloram 0.1 g L-1 + thiamin 0.2 g L-1 + IAA
1 g L-1 + kinetin 0.1 g L-1. In Sukma papaya seed varieties treatment with cryoprotectants of
PVS2 submersion for 30 minutes and the rate of 11 -13% water content provides the best
germination value (DB), the maximum growth potential (PTM), and the better rate of growth
(KCT), 38.39%, 38.39%, and 2:24%, respectively. | id |
