Comparison of Two Different Method for Sperm Concentration Measurement of Ram and Buck Semen

Abstract

Sperm concentration is the number of sperm cells contained in one milliliter semen. Careful evaluation and validation of sperm concentration is very important in artificial insemination technology, because an accurate estimation of concentration will determine extension rates that optimize farm animal’s utilization and fertility of extended semen. Ram and buck had a high sperm concentration and low in sperm volume. The concentration of the sperm can be calculated in various ways, from simple technique to see the distance between the head and to use computerize assisted sperm analysis. This research aimed to compare the concentration of buck and ram sperm using a counting chamber and photometer SDM6. Ten animals consist of 5 sexually mature rams and 5 bucks used as sperm source. Semen was collected using artificial vagina. Each individual semen were evaluated for sperm concentration using counting chamber (Neubauer improvement) and photometer SDM6 (Minitub, Germany). Result demonstrated that sperm concentration of buck and ram semen was not differ between photometer SDM6 and counting chamber (p<0.05), the sperm concentration of ram and buck semen were 4864.17±2044.92106 and 5432±1350.44 106 per mL respectively. In conclusion photometer SDM6 can be used as a new method for sperm concentration assessment with a faster time and accurate as counting chamber