Identification of Genotype DNA Microsatellite in Association with Performance of Indonesian Aceh Cattle

Abstract

The objectives of this research were to evaluate polymorphism of microsatellite DNA through the investigation of microsatellite loci BM1824, SPS115 and ILSTS028 and its possible association with body weight of Aceh cattle. A total of 126 head of DNA samples was collected from 3 local Aceh cattle population, i.e. Banda Aceh, Indrapuri and Saree district. Genetic polymorphism among group of Aceh cattle was calculated in frequency of alleles and genotypes. Association between genotypes and body weight was calculated by general linear model (GLM). Result showed that three loci showed high polymorphism. BM1824 locus had 13 genotypes with 3 highest frequency for BC (31.75), CC (19.84) then BB (14,29),while frequency of the other 10 genotypes providing AB, AC, AD, AF, BD, BE, BF, CD, DD and DF were less than 10 %. SPS115 locus had 10 genotypes with two highest frequency for CE (25.00) and BE (19.23), while the other 8 genotype were less than 14%. ILSTS028 locus had 28 genotypes with three highest frequency for CH (24,37), CC (11,76) and HH (10,08), respectively. It could be verified that Aceh cattle possessing typical genotypes of AF in BM1824 locus and AB in SPS115 locus was apparently related with a higher body weight compared to another genotypes, on the contrary, those possessing BB and BL genotypes in ILSTS028 was seemingly associated with a lower body weight