| dc.description.abstract | This aim research was to investigate the best soy extract concentration on the quality of Frisian Holstein (FH) bulls chilled semen.Semen were collectedfrom 5 FH bulls belong to Lembang Artificial Insemination Centre using artificial vagina. Immediatly after colection the semen evaluate macro-and microscopycally. Semen samples with >75% progressive motility and contained <20% sperm with abnormal morphology were used in the experiment. Semen individually divided into 4 tubes and diluted with 2.5%, 3.75%, 5%, or 6.25% Tris soy extract (TSE), diluted semen than store at 5 °C. Sperm motility and viability were evaluate once a day. Theresults showed that sperm diluted in2.5% TSE was the highest sperm motility (30.25±8.45%) at 72 hours of storage (day 3) than in 3.75% (18.00±5.12%), 5% (7.17±2.32%) or 6.25% (4.45±1.02%) TSE. No differences wasfound on the viabiliy of sperm, between allfour TSE concentrations at 0 and 12 hours of storage. At 24 until 72 hour of storage, semen diluted with 3.5% TSE significantly higher than other concentration. This research conclude that sperm motility diluted in 2.5% soy extract better than other concentration, while 3.75% soy extract maintain sperm viability better than other concentration. | id |
