Breeding, Clonal Propagation and Application of Molecular Marker Analysis of Phalaenopsis

Abstract

Phalaenopsis is one of famous orchid with high economic value. Breeding is needed to develop a new population as material selection for a new variety which adaptive to climate change. Molecular marker is useful for diversity analysis and molecular assisted breeding in plants. Phalaenopsis has long juvenile stage for flowering, in that case, molecular marker could be developed for early selection of progenies which have specific character. Clonal propagation is needed to provide the propagules of selected varieties. The research was carried out to collect and characterize some species and hybrids of Phalaenopsis, develop some hybrid population, clonal propagation or mlcropropagation protocol and apply molecular marker for diversity analysis of species and hybrids. ln this article, morphological and molecular characterization of TSW 1102 Phalaenopsis hybrid population (Phal. bellina x Phal. 'Salu Spot’) and clonal propagation of TSW 1103 Phalaenopsis hybrid population (Phal. 'Sogo Diamond' x Phal. 'Jin Bao Red Rose’) are reported. The results of the research showed that 123 progenies of TSW 1102 population were clustered into main group with the level of similarity about 50 or diversity 50%. Molecular marker analysis by using 3 locus of SNP marker which was developed from Chalchone Synthase Gene (CBS) Sequence could separate the population Into 3 group with the level of similarity 80-86%. Clonal propagation of TSW 1103 population carried out using axllary branchlng. Murashige and Skoog Medium wlth additlon of 10.30 ppm of benzylaminopurine (BAP) gave the best result.