Increasing Amount And Entrapment Efficiency Of Chitosan-Ketoprofen Nanoparticle Using Utrasonication Method With Varied Time And Amplitude

Abstract

Chitosan nanoparticles loaded by ketoprofen have been prepared by sonication and centrifugation methods. Preliminary tests have been carried out by conducting optimization condition of sonication including amplitude and sonication time. Turbidity data showed that the optimum condition for sonication on amplitude and sonication time at the percentage of 20% and at 60 minutes, respectively. PSA analysis indicated that decreasing of turbidity number of emulsion was also reduced particle size. PSA results showed that all formulas have a uniform particle size up to 100% with polydispersity index was below 0.3. B formula has smaller particle size and highest entrapment efficiency value than two formulae others, 222,1 nm and 87,463%, respectively. SEM and XRD analysis showed that chitosan nanoparticles loaded by ketoprofen have spherical form and semi crystalline properties, respectively.