Actinokineospora ba/iensis sp. nov., Actinokineospora cibodasensis sp. nov. and Actinokineospora cianjurensis sp. nov., isolated from soil and plant litter

Abstract

Six actinomycete strains isolated from soil and plant-litter samples in Indonesia were studied for their taxonomic position by using a polyphasic approach. Phylogenetically, all the strains were l~ted in the broad cluster of the genus Actinokineospore. Chemotaxonomic data [cell-wall diamino acid, meso-diaminopimelic acid; cell-wall peptidoglycan, type Ill (A 1 y}; major sugars, galactose and arabinose; major menaquinone, MK-9(H4); major fatty acid, iso-C16:0 ; major phospholipid, phosphatidylethanolamine) supported the affiliation of all six strains to the genus Actinokineospora. The results of DNA- ONA hybridization with DNA from type strains of Actinokineospora species with validly published names revealed three ONA-DNA relatedness groups. Group I (1003-0561 1) showed low relatedness to the other strains studied. The three strains in group II (1003-0784 r, 1003-0808 and 1003-0809) formed a group with high relatedness (98-100 %) and showed low relatedness to the other strains studied. The two strains in group Ill (1003-08101 and1003-0813) showed 58- 68% relatedness to Actinokineospora terrae NBRC 156681 and showed low relatedness (2-24%) to the other strains studied. The description of three novel species is proposed: Actinokineospora baliensis sp. nov., for the single strain in group I (type strain 1003-0561 1 =BTCC B-5541 =NBRC 1042111), Actinokineospora cibodasensis sp. nov., for the strains in group II (type strain ID03-07841 =BTCC B-5551 =NBRC 1042121) , and Actinokineospora cianjurensis ap. nov., for the strains in group tu (type strain 1003-08101 =BTCC 8-558T =NBRC 1055261).