| dc.description.abstract | Tomato chlorosis virus (ToCV), a whitefly-transmitted and phloem-limited Crinivirus infecting tomatoes worldwide, is reported for the first time occur in Indonesia in 2006. In tomato, ToCV cause interveinal yellowing that developed initially on lower leaves and then progressed to the upper part of the plant. Affected plants were less vigorous and yielded less due to reduced fruit growth and delayed ripening. Polyclonal antiserum has been already produced using capsid proteins of ToCV expressed in Escherichia coli. Reaction and titer of the antiserum were analyzed in this research. Serological reactions were analyzed using agarose gel precipitation test (AGPT) and dot immunobinding assay (DIBA). The titer of the antiserum was measured using undiluted sap prepared from ToCV-infected tomato leaf tissues. Based on the research result, the ToCV antiserum was specifically reacted with ToCV antigen. AGPT and DIBA gave a strong positive signal if the antiserum was reacted with ToCV-containing sap, but there no any positive signal if the antigen extracted from healthy tomato plant. Using AGPT method, the titer of the antiserum was only 1/2, but using DIBA the titer was reach up to 1/10000. The DIBA, therefore, considered as a reliable serological method for the ToCV using this antiserum. | en |
