Pengembangan Metode Deteksi Cepat Aspergillusjlavus Link. dan Fusarium sp. pada Benih Padi Menggunakan Laser-Induced Fluoresence

Abstract

Fungi is known to produce specific metabo!ites as its secondafy characters. The using of laser-induced fluoresence needs to be developed because each metabolite has specific fluoresence emission when exposed to near ultraviolet or ultraviolet light. Detection method for fungal diseases using laser-induced fluoresence has some advantages: fast, cheap, easy, and accurate. This study is aimed to develop rapid detection protocol by laser-induced fluoresence mechanism. The result of the this study is expected in the future fluoresence spectroscopy using laser light could be used as easy, cheap, fast, and accurate seed health testing method. The research was conducted in Micology Laboratory of Plant Protection Department and Biophysics Laboratory of Physics Department, Bogor Agricultural Institute, started from April 2008 until November 2008. This research covered (I) research preparation to obtain pure isolates of A. flavus and Fusarium semitectum Berk. & Rav., (2) calibration of laser-induced fluoresence which covered measurement wavelength of metabolite produced by A. flavus and Fusarium sp.; and fluoresence spectroscopy calibration of metabolite of A. flavus and Fusarium sp. as standard fungi metabolite, and (3) detection of A. flavus and Fusarium sp. on rice seed which covered fungi growth media modification as standard media to measure the wavelength of fungi metabolite; and laser-induced fluoresence application to detect A. flavus and Fusarium sp. The result of this study shows that metabolite of A. flavus produces blue fluoresence at 424-427 nrn, and also metabolite of Fusarium sp. produces blue fluoresence at 427 nm. A. flavus and Fusarium sp. display typical fluoresence spectra which differ from fluoresence spectra of growth medium PDA. In conclusion, specific fluoresence spectra have been identified which permit detection method of fungal infection on rice seed using laser-induced fluoresence