Karakterisasi Morfologi dan Biokimia Aksesi Pamelo {Citrus maxima (Burm.) Merr.} Berbiji dan Tidak Berbiji Asli Indonesia

Abstract

The objective of this work was to evaluate morphological and biochemical (isozyme) charaters of seeded and seedless pummelo accessions originated from Sumedang. Pati, Kudus and dan Magetan. Morphological characters contributed in grouping pummelo accessions were epicarp thickness, leaf lamina margin, vesicle length, epicarp color, petiole wing width and fruit shape, while isozyme characters were MDH (Rf 0,11 and 0,14) and ACP (Rf 0,24 and.0,33). ACP band at Rf 0,24 could be used as marker to differentiate seeded and seedless pummelo accessions. Separation between seeded and seedless accessions based on morphological characters occured at similarity coefficient of 0,63, while on isoenzyme characters occured at similarity coefficient of 0,49. Dendrogram based on combined morphological and isoenzyme data was able to differentiate seed bearing and seedless pummelo accessions. Principal component analysis results was congruent with that of morphological, isozyme, and combination of them.