Anatomy, Phisiology and Pharmacology: Recent submissions
Now showing items 61-80 of 139
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Normal Calves Obtained after Direct Transfer of Vitrified Bovine Embryos Using Ethylene Glycol, Trehalose, and Polyvinylpyrrolidone
(1996)In the present study, IVF bovine embryos were vitrified using as the cryoprotectants, ethylene glycol plus trehalose plus the polymer, polyvinylpyrrolidone (PVP). In Experiment I, toxicity of the vitrification solution ... -
Production Of Chimeric Calves Derived From Parthenogenetic And Fertilized Bovine Embryos
(1993)The present study was aimed to examine whether parthenogenetic cells could contribute to the development of bovine embryos to term. Chimeric blastocysts were produced by aggregation of parthenogenetic and in vitro fertilized ... -
The Role Of Pre-Equilibration For Vitrification Of In Vitro Produced (Ivp) Bovine Embryos
(1993)The role of equilibration for successful vitrification was investigated by one-step or three-step addition ard one-step removal of the cryoprotective agents (CPAs) in vitrification solution. The blastocysts ard expandedblastocysts ... -
Calves Obtained After Transfer Of Frozen-Thawed Bovine Immature Oocytes Cryopreserved In Various cryoprotectants
(1993)The objective of this study was to evaluate the fertilization and cleavage rates in vitro of frozen-thawed bovine oocytes at the germinal vesicle (GV) stage. Various permeating cryoprotective agents [1.8M ethylene glycol ... -
The Influence Of Sperm-Oocyte Incubation Time And Breed Of Bull On In Vitro Embryo Development In Catile
(1993)It has been reported that the length of sperm-oocyte-incubation time using Holstein sperm did not affect to the embryo development (Rehman, et al. Theriogenology 41:1447- 1452, 1994). Therefore, the present study was ... -
Aggregation of parthenogenetic and fertilized embryos for producing chimera calves
(1993)Chimeric blastocysts were produced by aggregation of parthenogenetic and fertilized demi-embryos from Japanese Brown breed and Holstein breed cattle, respectively. The fertilized embryos were obtained by in vitro maturation, ... -
Vitrification of bovine in vitro matured and pronuclear oocites with different vitrification solutions
(1993)Vitrification of in vitro matured (IVM) and pronuclear (PN) oocytes was performed using three types of vitrification solutions (VS). IVM oocytes were vitrified with precooled (4°C) VS containing 40% ethylene glycol + 0.3M ... -
Development Of A Simple, Portable Carbon Dioxide Incubator For Production Of Bovine Ivf Embryos
(1993)We have developed a simple, economical and portable C02 incubator for production of bovine IVF embryos in the field. The incubator consists of a metallic chamber (29L x 26W x 21 H em); the bottom plate of this chamber is ... -
Viability Of Bovine Blastocysts Obtained After 7, 8 Or 9 Days Of Culture Following Vitrification And One-Step Rehydration
(1993)This study examined the morphological appearance, hatching rates and live:dead cell ratios following vitrification of in vitro produced (IVP) bovine blastocysts. Expanded blastocysts obtained after 7, 8 or 9 d of culture ... -
Effect Of The Presence Of A Cl In The Ovary On Oocyte Number, Cleavage Rate And Blastocyst Production In Vitro In Cattle
(1993)High progesterone concentration has been shown to cause regression of the dominant follicle and follicle wave turnover in cattle (Taylor and Rajamahendran, can J Anim Sci 74:281,1994). Pregnant cows also produced a higher ... -
Viability Of Frozen-Thawed Bovine Ivm/Ivf Embryos In Relation To Aging Using Various Cryoprotectants
(1994)Bovine IVF embryos developed on Days 7, 8 and 9 were equilibrated with 1. 6 M propylene glycol (PG) , 1. 8 M ethylene glycol (EG) , 1. 1 M diethylene glycol (DEG) or 1 . 3 M ethylene glycol monomethyl ether (EME) for 10 ... -
Production of chimeric calves by aggregation of in vitro-fertilized bovine embryos without zonae pellucidae
(1993)Bovine embryos produced by in vitro maturation (IVM). fertilization (IVF) and culture (IVC) were used to produce aggregation chimeras. An aggregated chimera was produced by combining bovine IVF embryos (Holstein x Japanese ... -
Pregnancy Rate And Survival In Culture Of In Vitro Fertilized Bovine Embryos Frozen In Various Cryoprotectants And Thawed Using A One-Step System
(1993)Bovine oocytes surrounded with compact cumulus cells were cultured for 20 to 22 hours (38.5°C, 5% C02) in modified TCM-199 medium supplemented with 5% superovulated cow serum (SCS) and inseminated by in vitro capacitated ... -
Pregnancy rate and survival in culture of in vitro fertilized bovine embryos frozen in various cryoprotectants and thawed using a one-step system
(1993)Bovine oocytes surrounded with compact cumulus cells were cultured for 20-22 h (38.5°C, 5 % C02) in modified TCM -199 supplemented with 5 % superovulated cow serum (SCS) and inseminated by in vitro capacitated sperm. Day ... -
Chimeric Blastocyst Production Using Ivf Bovine Embryos (Holstein-Japanese Red} Without Zona Pellucida
(2012)The objective of this study was to produce chimeric blastocysts by the aggregation of IVF bovine embryos (Holstein- Japanese Red}, without zonae pellucidae. The embryos were produced by the in vitro maturation and fertilization ... -
Selected single blastocyst transfers maintained pregnancy outcome and eliminated multiple pregnancies
(2004)Background: Transfer of more than one embryo following in vitro fertilization/intracytoplasmic sperm injection cycles have increased pregnancy rate at the cost of increasing the incidence of triplets and twins. It has been ... -
Offspring born from chimeras reconstructed from parthenogenetic and in vitro fertilized bovine embryos
(1999)Chimeric embryos were produced by aggregation of parthenogenetic (Japanese Red breed) and in vitro fertilized (Holstein breed) bovine embryos at the Yamaguchi Research Station in Japan and by aggregation of parthenogenetic ... -
Transfer of Inner Cell Mass Cells Derived from Bovine Nuclear Transfer Embryos into the Trophoblast of Bovine In Vitro–Produced Embryos
(2006)Presence of placental tissues from more normal noncloned embryos could reduce the pregnancy failure of somatic cloning in cattle. In this study, inner cell mass (ICM) cells of in vitro–produced (IVP) embryos was replaced ... -
The effect of sperm-oocyte incubation time on in vitro embryo development using sperm from a tetraparental chimeric bull
(1997)The present study was designed as 5 X 4 factorial to investigate the effects of using sperm from 5 bulls, and varied sperm-oocyte incubation times (5, 10, 15 and 20 h) on the fertilization, cleavage rates and blastocyst ...
