Kajian Sumber Cemaran Mikrobiologis Pangan pada Beberapa Rumah Makan di Lingkar Kampus IPB Darmaga, Bogor

Abstract

Food borne diseases by microorganism was still happened as a lot of cases per annum. Sources of food microbiological contamination were raw materials, personal hygiene, sanitary of equipment and airborne and water sources. The goal of this research was to study of sources of food microbiological contamination in five restaurants around IPB campus which sold "pecel ayam". Microbiological testing was conducted by total plate count, most probable number of coliform and biochemical identification of Salmonella sp. and Escherichia coli. Personal hygiene and sanitaryof water and equipment testing have been done to detect sources of microbiological contamination. Food without heat treatment (cucumber) has a large amount of microorganisms (2.9-6.8 log CFU/g) and coliform (2.5-3.1 log MPN/g). Smoked and fried chicken have total microorganisms under 1.4 log CFU/g and total coliform under 0.3 log MPN/g. Sambal have total microorganisms between 1.4-4.1 log CFU/g and total coliform between 0.3-4.1 log MPN/g. Neither Enterobacter aerogenes, nor Escherichia coli, the bacterium could occasionally cause gastroenteritis, was identified from all of the smoked and fried chicken. E. coli has been isolated from cucumber of RM3, and the best possibility source of this bacterium was water source. Source of Enterobacter aerogenes which identified from cucumber and sambal were personal handed, talenan and water source. Salmonella has not been identified from the entire sample.