Isolation and introduction of citrate synthase gene of pseudomonas aeruginosa into plant to increase aluminum tolerance

Abstract

Commonly, marginal land was known as acid soil with alumunium toxicity as the main problem. Nevertheles, acid soil has the potential for expansion of agricultural lands both for food crops and energy producing plant. Our research target was to develop a biodiesel plant, Jatropha curcas, which has a good growth performance in acid soils. This research divided into four steps: 1) analysis of J curcas response to A1 stress, 2) isolation and chararacterization of Pseudomonas aeruginosa citrate synthase gene (PaCS), 3) expression of PaCS into model plant, Nicotiana tabacum, and 4) overexpression of PaCS gene in J. curcas.