The effect of Ambon Banana Stem Sap (Musa paradisiaca forma typica) on the Acceleration of Wound Healing Process in Mice (Mus musculus albinus)

Abstract

The aim <!(this research is to find out the ac1ivi(l' of banana stem sap (Musa paradisiaca_limna typica) on the acceleration ofwmmd healing process in lhe mice skin (Mus musculus albimts) hosed on g.-ass and histopathological observations. Total(\' 45 heads ofDDY mire ages 4-6 weeks were used in this sllldy. The mice were dh·hled into three groups, negative control (without treatment), positiPe control 1Bioplacenton ') and hanana stem sap. All mice were 1-1.5 em incised on the dorsal back skin. Gross lesions were obsen''!d dai(v. On the .3.-.~, 5'h, 1h, 14'h and 21"' days c!fier the treatment, mice were elllhanb.>d and the skin samples were collected for fitrther histopathological observation. The anatomical parameters were blood coagulation, dryness, alluchment/narrowed of the wound andformation of the blood clot. The histopathological parameters were number of macrophages, neutrophils, (vmphocytes, neo-1•ascularisation percentage ofre-epithelization and the thickness of fil>roblast. All qualitalii'P data were statistical~v anu(v::ed using Ana{v.fis of Variance (ANOVA) and continued with Duncan Multiple Range Test. Gross lesion and the fihroblastthickness were ob.~erved and descriptively analyzed a.~ a quantitative data. 17te result indicated that hanana stem sap could promoie the wound healing process. Gross lesion ol>servation indicated that in the l>anana stem sap treatment the scab .fhrmation was fmter than negative control and Bioplacenton~. On macrophages, neutrophil~ and (1'111phoc.1·tes al>serPations, the stati.;tical ana{vze showed that the banana stem sap treatment was significant increase (P<O.IJ5) than the negatil'e control. The {ibroblmt thickness on the skin wound treated with banana stem sap was high a~d the formation was also faster than the negative control and Bioplacenton''. All result mentioned above indicated that Ambon banana stem sap was accelerated the wound healing process. Further study is required in order to c/ar!f}• the mechanim1 of the sap on wound healing process as well as thir toxicity and possibility for use both in animal and humattmedicine.