View Item 
      •   IPB Repository
      • IPB e-Journal
      • Jurnal Hemera Zoa
      • View Item
      •   IPB Repository
      • IPB e-Journal
      • Jurnal Hemera Zoa
      • View Item
      JavaScript is disabled for your browser. Some features of this site may not work without it.

      Deteksi penyakit TSV (Taura Syndrome Virus) secara PCR udang vannamei (Litopenaeus vannamei) dengan berbagai ekstraksi, suhu dan waktu penyimpanan

      No Thumbnail [100%x80]
      View/Open
      Full Text (915.8Kb)
      Date
      2010
      Author
      Surfianti, O.
      Prihartini, N.C.
      Fathoni, M.
      Ekoputri, E.R.
      Laminem
      Wilis, R.
      Pujiastuti, E.
      Sokhib
      Koswara, A.D.
      Metadata
      Show full item record
      Abstract
      The shrimp farming industries have been adversely affected by epizootics due to viral pathogens, especially Taura Syndrome Virus (TSV The TSV has been the causative agent of economically disastrous epizootics in Penaeus vannamei, causing mass mortalities of 40% - 90% in affected post larval and juvenile population. The current diagnostic and detection methods for TSV included clinical signs, gross pathology, in situ hybridization, and PCR. Three RNA extraction methods, i.e. RNA lysis buffer with Guanidine- HCL, RNA lysis buffer with beta-mercaptoethanol dan phenolchloroform) were used to evaluate the vannamei shrimp infected with TSV The study showed that RNA extracted using the RNA lysis buffer with beta-mercaptoethanol, constantly had the highest yield as measured (quality and quantity) using a geneQuant spectrophotometer at period of 3 mo11th in -20 OC storeage, except the one extracted by phenol - chloroform extraction had the highest yield quantity at -80 'C. Each of 3 (three) extraction methods yielded sufficient RNA for positive results in a RT PCR for TSV at period of 1 mo;zth,and 2 months, in both tempaature storage (-20°C and -800C), but at period of3 months, only the phenol- chloroform extraction give positive result after it was stored at -20°C and -80 °C.
      URI
      http://repository.ipb.ac.id/handle/123456789/59407
      Collections
      • Jurnal Hemera Zoa [9]

      Copyright © 2020 Library of IPB University
      All rights reserved
      Contact Us | Send Feedback
      Indonesia DSpace Group 
      IPB University Scientific Repository
      UIN Syarif Hidayatullah Institutional Repository
      Universitas Jember Digital Repository
        

       

      Browse

      All of IPB RepositoryCollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

      My Account

      Login

      Application

      google store

      Copyright © 2020 Library of IPB University
      All rights reserved
      Contact Us | Send Feedback
      Indonesia DSpace Group 
      IPB University Scientific Repository
      UIN Syarif Hidayatullah Institutional Repository
      Universitas Jember Digital Repository
        

       

      NoThumbnail