Pengaruh Suhu Rekonstitusi Terhadap Isolat Lokal Enterobacter sakazakii (Cronobacter sp.) Asal Susu Formula dan Makanan Bayi

Abstract

Enlerobacter sakazakii (recently known as novel genus Cronobacter sp.) is opportunistic bacteria which can cause severe meningitis in neonates. Eight isolates of E.sakazakii which previously isolated from powdered infant formula (PIF) and weaning food were tested for their ability to survive during reconstitution with water having various temperatures, and their fate during hang time in comparison with 7 isolates previously described by Estuningsih and E.sakazakii ATCC 352/7. Reconstitution with 100°C water decreased the number of bacteria of most isolates to undetectable level, while those with 40°C and 4°C water did not reduce the bacterial number significantly. Using water of 70°C, reconstitution decreased the number of bacteria of 10 isolates to undetectable levels: however 6 isolates survived the reconstitution temperatures. The hang time test showed that some bacteria which were not detected after reconstitution with 70°C water became detectable after 2 hours. Those surviving reconstitution with 70°C grew well during hang time for 2 to 8 hours.