The determination of asiaticoside content and screening of acetylcholinesterase inhibitory potency of gotucola (Centella asiatica) harvested from different location

Abstract

Gotu cola (Centella asiatica) with characterized coumpound was reported to have a potential anti dementia effect by modifying acetylcholinesterase activity (Ach) The activity of the gotucola to Ach may vary according to the place where gotucola is cultivated. This research was focused on the determination of asiaticoside content and screening of acetylcholinesterase inhibitory potency of gotu cola collected from different location namely Pacitan, Wonogari (a), Wonogiri (b), Solo, Wonosobo, Karanganyar and Bogor. Asiaticoside contents of gotucola was determined by HPLC method and inhibitory activity was determined by colorimetric method modified version of Ellman (Perry et al 2000). The result showed the three location of collected gotucola which have highest concentration of asiaticoside namely Wonogiri (b) , Solo, and Wonogiri (a) with range concentration per gram extract were obtained from 10.53 mg ,9,4 mg and 8.9 mg of respectively. In the point view of the inhibitory activity of extract on acetylcholinesterase, the three locations that have gotucola exctracts with hight potency as acetilcholinesterase inhibitor are wonogiri (a), Karanganyar and Pacitan with the percentase inhibitions are 62.16%, 59.5 %,and 56.8 % respectively. Based on asiaticoside content of different location from Wonogiri ( a and b) and its inhibitory activity on acetylcholinesterase, inhibitory activity of extract on acetylcholinesterase may not correlate with amount of asiaticosid content.