In Vitro Regeneration of Pummelo cv. Cikoneng from Cotyledon and Epicotyl

Abstract

The objective of the present research was to obtain an efficient and reproducible regeneration protocol from cotyledon and epicotyl. Experiment was arranged in a factorial design with two factors, replicated 20 times. The first factor was type of explant, i.e. cotyledon and epicotyls segments of Pummelo cv. Cikoneng while the second factor was the media. The media were as follows: (1). MS + 1.0 mg BAP/l + 0.5 mg K/l + 0.5 mg NAA/l. (2). MS + 2.0 mg BAP/l + 0.5 mg K/l + 0.5 mg NAA/l; (3). MS + 1.0 mg BAP/l + 1.0 mg K/l + 0.5 mg NAA/l; (4). MS + 2.0 mg BAP/l + 0.5 mg K/l + 1.0 mg NAA/l; (5). MS + 2.0 mg BAP/l + 1.0 mg K/l + 1.0 mg NAA/l. Four cotyledons (1.0 - 1.5 cm) were placed abaxially and 5 epicotyl segments (1.0 cm) were placed horizontally on the medium in each culture jar. Observation was made on number of days to induce shoot number of explant forming shoots, shoot heigh, number of shoots, leaves and roots. Result of the study showed that treatment of cotyledon explant culture in media 1 or media 3 gave the highest number of adventive shoots, number of leaves and number of roots. Those combinations of treatment were the fastest in shoot initiation. It is suggested that cotyledon and media 1 is used for pummelo propagation.