Regenerasi Terubuk (Saccharum edule Hasskarl) Secara In Vitro

Abstract

Terubuk is one of the potential plant for vegetable known as sugarcane cauliflower. Production need cutting material or propagules. The objective of this research was to obtain the best method to propagate terubuk using in vitro micropropagation through direct and indirect organogenesis. Flower stalks were used as explants. The indirect organogenesis using calli induction showed that the best medium was MS + 3 mg l-1 2,4-D + 1 mg.l-1 kinetin. This media did not produce shoots from calli proliferation stage, only able to produce roots. The direct organogenesis showed that the best medium was MS + 0,25 mg l-1 thidiazuron + 0,1 mg l-1 NAA + 0,25 mg l-1 GA3 to produce shoots in 2 weeks without going through calli induction medium. Root formation required full strength of MS salt.