Ability of cajuput candy in maintaining oral health: in vitro inhibition of cajuput candy flavor formula against streptococcus mutans and streptacoccus sobrinus

Abstract

Streptococcus mutans and Streptococcus sobrinus are microorganisms commonly ---- and isolated from human mouth. The c serotype of S. mutans is a major pathogen causing dental caries in human. Cajuput oil has been reported for having antiseet compound which being able to prevent dental caries. Cajuput oil contains 10% crystal phenolic compound, 3,S-dimethyl-4,6-di-0-methylphloroacetophenon, which known anti-microbial compound. This research aims to identify the ability of cajuput candy prevent biofilm formation by Streptococcus mutans and Streptococcus sobrinus in ---- and observing the effect of cajuput oil inhibition ability in different concentration. the research has been conducted in vitro, by incubating the bacteria on 96 well tissue culture plates and then each well was added with the tested compounds. The analyst was done according to the method of Yamanka. There were 6 formulations of cajuput candy formula which had been observed, consisting of combined flavors as well as single flavor. Hedonic sensory evaluations were done to observe consumer acceptance --- various cajuput oil concentrations those have been proven could inhibit the biofilm formation of S. mutans and S. sobrinus. Administration of cajuput candy formulation do not increase the biofilm formations by c serotype of S. mutans, whereas, it tended to reduce the film formation comparing to control. Cajuput candy formulation, however could not suppress biofilm formation by d serotype of 5. mutans.Peppermint oil as single flavor ingredien offered higher protection compared to cajuput oil. However, maximum protection against the tested microbes was achieved by combined flavor compound than its single flavor compound. The inhibition of cajuput oil concentrations on biofilm formation was concentration dependent, the higher the better.lt showed that 0.5 concentration of cajuput oil has already been able to inhibit the biofilm formation by and d serotype of S. mutans.The most accepted formulation with potential activities --- hedonic score about 6.91(slightly like to like) for aroma and 6.77 (slightly like to like) --- taste.This formulation has significant difference result compared with the other formulations.