In Vitro Anti-Proliferation and Anti-Invasion Activities of the Combination Between Recombinant Canine Interferon (rCaIFN) with Luffa cylindrica Seed Methanol and Chloroform Extracts on MCM-B2 Derived Tumor Cell Line In Collagen Gel Medium

Abstract

In vitro antiproliferation and anti-invasion activity of the combination between recombinant canine interferon (rCalFN) and Luffa cylindrica seed methanol and chloroform extracts on MCM-B2 tumor cell lines was studies. The dose of the extract was 100 ppm for the chloroform exctract extract and 350 ppm for the methanol extract, while the dose of rCaIFN was 10⁴ IU/ml. The highest anti-proliferation activity of the methanol extract- rCaIFN combination was 84% while the chloroform extract- rCaIFN combination was 60%. The anti-invasion activity was detected on the semi solid medium of collagen gel on both extract combinations even there was a variation on the inhibition capacity among them. The methanol exctract gave the highest inhibition of the cell invasion activity. The ability of the inhibition of cell invasion activity was similar to that of anti_proliferation activity. The result of the present study indicated that the combination of Luffa cylindrica and rCaIFN have a sysynergetic effect on the inhibition of cell invasion and we concluded that this combination give a promising hope for the tumor disorders treatment. The mechanisms of this combination activity is still unclear and under investigation.