Design and Application of a New Cryptic-Plasmid-Based Shuttle Vector for Magnetospirillum magneticum
Date
2003Author
Okamura, Yoshiko
Takeyama, Haruko
Sekine, Takumi
Sakaguchi, Toshifumi
Wahyudi, Aris Tri
Sato, Rika
Kamiya, Shinji
Matsunaga, Tadashi
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A 3.7-kb cryptic plasmid designated pMGT was found in Magnetospirillum magneticum MGT-1. It was characterized and used for the development of an improved expression system in strain AMB-1 through the construction of a shuttle vector, pUMG. An electroporation method for magnetic bacteria that uses the cryptic plasmid was also developed