Perkembangan oosit kambing setelah maturasi fertilisasi dan kultur in vitro

Abstract

Developmental competence of caprine oocyte after in vitro maturation, fertilization and culture was evaluated in this study. Oocytes were collected from ovaries obtained from local slaughterhouse by aspiration of the follicles (2 to 5 mm diameters) followed by slicing the ovaries. Collected oocytes were incubated in maturation medium for 18, 22, 26 and 30 hours. The viability and motility of sperm fresh and frozen-thawed) in fertilization media (Brackett and Oliphant medium, BO and TCM-199) were examined after 0, 3, 6 and 24 hours of incubation. Matured oocytes were fertilized with 5 x 106 sperm per ml offresh ejaculate for 18 hours followed by cultured in vitro for further development. Ten oocytes per ovary were collected from caprine ovary by aspiration method, and the additional 8 oocytes per ovary followed by slicing method. The maturation rates (metaphase II stage) were observed 36.59%, 78.26%, 88.00% and 83.12% after incubation for 18, 22, 26 and 30 hours, respectively. The viability of frozen-thawed sperm were lower than fresh ejaculate both in BO or TCM-199 media on 0 hour of incubation. The viability and progressive motility of frozen-thawed sperm were decreased after 6 hours of incubation and they were no sperm motil after 24 hours of incubation. However, the viability and progressive motility of fresh ejaculated sperm were maintained until 24 hours of incubation. Fertilized oocytes were developed to cleavage (47.00%) and morula/blastocyst (38.52%) .stages after culutre in vitro. These results show that caprine embryo could be produced after in vitro maturation. fertilization and culture.