Chimeric blastocysts produced by aggregation between parthenogenetic and fertilized bovine embryos

Abstract

Chimeric blastocysts were produced by aggugation of parthenogenetic and fertilized embryos.The normal fertilized embryos were obtained by in vitro maturation, fertilization and culture procedures.To produce parthenogenetic activation, matured oocytes were treated with 7% ethanol followed by 5 µg/ml cytochlllllsin D and cultured at 385oC under 5% CO2 in air. Aggregation of embryos were produced by: (1) injection of 2 blastomeres of parthenogenetic embryo (16-cell stage) into fertilized embryo (4-all stage), (2) injection of parthenogenetic demi-embryo (8-cell stage) into fortilized demi-embryo (8-cell stage),(3) in jed ion offmilized dnni-mabryo (8-all slllge) into parthenogenetic demi-embryo (8-all slllge) and (4) aggregation of whole parthenogenetic and fertilized embryo.The developmental rate of aggregated embryos produced by aggregation of whole embryos UGS significantly higher ( P<0.05) than by injections.Thirteen aggregated embryos were karyotyped,resulted on eight of embryos had XX and XY chromosome plate in the Same sample.These results onifiLd thllt the parthenogenetic cells can be contributed to the development of the embryo.