Prospek pemanfaatan telur ayam berkhasiat anti virus avian influenza dalam usaha pengendalian infeksi virus flu burung dengan pendekatan pengebalan pasif

Abstract

Production of polyclonal antibody against avian influenza type H5N1 and H5N2 was done in horse, cavia and chicken using respective commercial avian influenza vaccine. The presence of specific antibody in sera as well as egg yolk was detected with haemagglutination inhibition test (HI) and agar gell pecipitation test (AGPT). One week after first vaccination the presence of specific antibody in chicken sera could be detected in HI test with titer 22-24 using homolog antigen. The titer value discrepancy of 1-2 digits was detected using hcterolog antigen. The titer of antibody increase significantly after booster treatment, in horse sera with HI value 24 , 25-29 in cavia and 25-27 in chicken sera. The purification of IgG and IgY was done using affinity chromatography technique. Cavia Ig G had neutralization ability to AI virus H5N1 isolate 2005 with the titer of 104 EID 50 was 1,3. This indicated that by the dilution of sera 101,3, could neutalize 50% of the AI virus with titer of 104 EID 50. Egg yolk containing specific antibody with the same titer could neutralize all viral particles used in the assay (100%). Using spray dried egg yolk containing antibody with titer 10IJ could neutralize 50% of AI virus 104 EID 50, and titer antibody of 101,5 neutralized 80% of AI virus 104 EID 50. These results indicated a good prospect of using chicken egg for the production specific antibody (IgY) against AI virus and could be used in the passive immunization.