Chemical Composition Response of Cocoa Pod Incubated with Phanerochaete chrysosporium on Manganese and Calcium Supplementation

Abstract

Bioconversion is a method to increase quality of high lignocellulose-containing feedstuffs. Fermentation occurs during bioconversion is infl uenced primarily by length of fermentation and mineral supplementation to the medium. This study was aimed at determining the effect of these two factors on dry matter (DM), organic matter (OM), neutral detergent fi ber (NDF) and acid detergent fi ber (ADF), and cellulose-to-lignin ratio of cocoa pod incubated with Phanerochaete chrysosporium. Twenty four treatments containing of 4 mineral supplementations (no mineral, Ca, Mn, and Ca+Mn) and 6 different lengths of fermentation (0, 5, 10, 15, 20, and 25 days) were designed randomly to 72 fermentation glass jars in a 4x6 factorial arrangement. Length of fermentation had signifi cant effect on all parameters measured. Mineral signifi cantly affected changes of DM and OM, NDF and ADF content, and cellulose-to-lignin ratio, but not DM and OM content. In conclusion, supplementation of Ca to cocoa pod incubated with P. chrysosporium for 15 days contributed positively (P<0.05) to changes of OM (13.83%) and DM (11.30%). The cellulose-to-lignin ratio of 1.34 was the optimum result of Mn supplementation for 10 days incubation.