dc.contributor.author | Lintang, Rosita | |
dc.contributor.author | Suhartono, Maggy Thenawidjaja | |
dc.contributor.author | Hwang, Jae Kwan | |
dc.contributor.author | Pyun, Yu Ryang | |
dc.date.accessioned | 2011-03-23T02:01:44Z | |
dc.date.available | 2011-03-23T02:01:44Z | |
dc.date.issued | 2007 | |
dc.identifier.issn | 1978-3477 | |
dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/42883 | |
dc.description.abstract | The thermostable chicken feather degrading protease enzymes used here was extracted and partially purified from thermophilic bacteria L-23 isolated from a coastal hot spring in North Sulawesi, Indonesia. The L-23 was grown in the selective medium containing 1% chicken feather powder at 70 °C and pH 7. The cell-free culture was precipitated with ammonium sulphate at 80% saturation, followed by heating at 65 °C for 1 h before applied onto Sephadex G-100. The molecular weight of the two enzymes identified were estimated as 47 and 64 kDa. The optimum pH of the mixed enzymes preparation was 7 while the optimum temperature was 65 °C. Zymogram analysis showed that one of the enzymes was still active after being heated at 100 °C for 20 min and was also resistant towards organic solvents and SDS. The activity was enhanced by addition of 1 mM FeCl3. | en |
dc.publisher | IPB (Bogor Agricultural University) | |
dc.relation.ispartofseries | Vol.1;No.3 | |
dc.title | Thermostable Chicken Feather Degrading Enzymes from L-23 Isolate from Indonesia | en |
dc.title.alternative | Microbiology Indonesia Vol.1 No.3 Tahun 2007 | en |