dc.contributor.author | Artika, I Made | |
dc.date.accessioned | 2011-03-22T04:06:17Z | |
dc.date.available | 2011-03-22T04:06:17Z | |
dc.date.issued | 2009 | |
dc.identifier.issn | 1978-3019 | |
dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/42742 | |
dc.description.abstract | The yeast Saccharomyces cerevisiae HIS6 gene has been shown to be functional as a selectable marker for selecting and maintaining a yeast vector in yeast S. cerevisiae host cells. The yeast HIS6 gene encodes an enzyme involved in the yeast histidine biosynthesis. The yeast HIS6 gene was cloned into a yeast expression vector. The resultant recombinant plasmid was introduced into yeast host cells defective in endogenous HIS6 gene. The functionality of the HIS6 gene as a selectable marker was tested by growing transformed cells on selective minimum medium lacking histidine supplementation. | en |
dc.publisher | IPB (Bogor Agricultural University) | |
dc.relation.ispartofseries | Vol 16;No 1 | |
dc.title | The use of HIS6 gene as a selectable marker for yeast vector | en |
dc.title.alternative | HAYATI Journal of Biosciences Vol. 16 No. 1 Tahun 2009 | en |