Injeksi Spermatozoa Domba Hasil Pengeringbekuan ke dalam Sel Telur Menggunakan Teknik Intracytoplasmic Sperm Iw'ection (IC SI)

dc.contributor.author	Saili, Takdir
dc.contributor.author	Setiadi, Mohamad Agus
dc.contributor.author	Agungpriyono, Srihadi
dc.contributor.author	Boediono, Arief
dc.date.accessioned	2011-03-08T04:01:32Z
dc.date.available	2011-03-08T04:01:32Z
dc.date.issued	2007
dc.identifier.issn	1411-8327
dc.identifier.uri	http://repository.ipb.ac.id/handle/123456789/42595
dc.description.abstract	In ths experiment, the ability offeeeze-dried ram spermatozoa to decondense and to support pronucleus formation after injection into oocyte using ICSI method was evaluated. Aceto lacmoid staining was used to assess decondensation and pronucleus formation following ICSI.' Results of these experiments revealed that freeze-dried spermatozoa had the ability to decondense (2%) and to induce IPN formation (34%) following injection into oocytes but fail to form 2PN. However, 30% of 2PN and 40% oocytes were obtained when oocytes were injected with fresh spermatozoa. In conclusion, freeze-dried ram spermatozoa were able to decondense and to support 1PN formation after ICSI. Key words: spermatozoa, freeze-dried, oocyte, ICSI, decondensation, pronucleus.	en
dc.publisher	IPB (Bogor Agricultural University)
dc.title	Injeksi Spermatozoa Domba Hasil Pengeringbekuan ke dalam Sel Telur Menggunakan Teknik Intracytoplasmic Sperm Iw'ection (IC SI)	en
dc.title.alternative	Jurnal Veteriner Vol.8 No.1 Th.2007	en