In vitro Selection For Drought Resistance On Rice

Abstract

The rice demand is recently increasing , on the other hand the production is decreasing. It is caused by the long dry season due to the seasonal change. In addition, a lot of productive land has changed into industrial and settlement which in return minimize the number of productive land. To overcome this 2 varients with high yield and drought-stress resistant as in IR 64 variety should be produced. In this attemt, mutative induction combined with in vim culture and in vitro selection on the Gajahmungkur, Topwuti and IR 64 variants has been conducted and has yielded several somaclones which show the genetic variation. Through evaluation and characterization for the drought resistant using PEG 20% (BM 6000), several somaclone assumed to be drught resistant have been obtained. Subsequently, to find , out the mechanisme of the respective genotype toward the drought stress resistance, root penetration and proline content analysis at the somaclone have been $ conducted. From the evaluation, 23 Gajah mungkur somacloncs, 9 Towuti somaclones and 13 Ir 64 somaclones have been obtained as the ones with the high proline content. The drought stress treatment to the assumed drought stress resistent it is obtained 9 somaclones from Towuti, 5 from Gajahmungkur and 8 from IR 64 with the better characteristics under drought test than that of the main plants. Shorter plant with more shoots are obtained from Gajahmungkur but the seeds ripe earlier. From Tohti variants, more shoots and higher plants along with the more grain are obtained. From variety of IR 64 , somaclone which can produces seeds during the drought is obtained, meanwhile this does not happen with the plant originated from seed. The plants resulted from the selection produce more qualified shoot and grain than that of the main plants.