Transformasi Genetik Tanaman Kentang cv. Atlantik Dengan Mengintroduksikan Gen Hordothionin untuk Mendapatkan Ketahanan terhadap Penyakit Bakteri

Abstract

Hordothionins are small anti-bacteria proteins present in barley endosperm. To reveal the potential of this proteins for engineering bacterial disease resistance into potato, a semi-synthetic hordothionin gene construct was introduced in potato cv. Atlantic via Anrobacterium tumefaciens strain LBA 4404, under the control of a caul~jlower mosaic virus (CaMV) 35s promotor. The in vitro grown stem (internodus) was used in this research. Afer 6 weeks in regeneration medium and 2 weeks in rooting medium there were 22 regenerated plants that were screened in kanamycine containing medium. PCR analysis using spesrfic primer from CaMV 35Spromotor showed the presence of ampl19ed T-DNA in 4 transgenic lines from 22 putative transgenic plants were tested. The in vitro toxicity against Ralstonia solanacearum tested from transgenic lines showed variation in resistance level. There were only 2 of the transgenic lines were tolerant, while one of them was moderate tolerant even one of them was susceptible.