Pemetaan Marker Aflp untuk Membuat Peta Genetik Bit Gula

Abstract

One hundred eighty two AFLP marker using primer combination of EcoRl/Msel and Pstl/Msel were used in this study to create a DNA marker genetic map of sugar beet. In average each primer combinationyielded 15.5 polymorphic band. AFLP marker using primer combination of EcoRl/Msel signifcantly yielkded more polymorphic band than Pstl/Msel.From this study a high dendity DNA marker map coverage all nine chromosomes of sugar beet with totally length of 744 cM Haldane was established.