dc.contributor.author | Tan, Irawan | |
dc.contributor.author | Suwanto, Antonius | |
dc.contributor.author | Suhartono, Maggy Thenawidjaja | |
dc.contributor.author | Purwadaria, Tresnawati | |
dc.date.accessioned | 2010-07-10T06:54:05Z | |
dc.date.available | 2010-07-10T06:54:05Z | |
dc.date.issued | 2001 | |
dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/32027 | |
dc.description.abstract | A xylanolytic-thermophilic bacterium (IT-08) was isolated from Gunung Pancar Hot Spring after two days of enrichment in Modified Thermus Medium (MTM) supplemented with 0.5% oat spelt xylan. 16s-rRNA sequence analysis indicated that IT-08 resembles Bacilius thermoleovorans, a species of thermophilic bacteria. When grown on xylan containing media, IT-08 produces a thermoactive xylanase. Xylanase from IT-08 was active at temperatures between 40 and 100°C, at pH values between 4.0 and 9.0 with opthum values obtained at 80°C and pH 6.0, respectively. SDS-PAGE and zymogram analysis demonstrated that a crude xylanase complex of IT-08 comprised two active bands with molecular masses of 78 and 60 kDa. | id |
dc.publisher | IPB (Bogor Agricultural University) | |
dc.title | Characterization of Xylanase from a Xylanolytic Thermophilic Bacterium Isolated from Gunung Pancar Hot Spring, West Java | id |