dc.description.abstract | The impact of extraction temperature, moisture content and storage temperature on in vitro pollen germination and tube growth, was determined in a sequence of experiments. Pollen extracted at 25°C, dried to 10% moisture content and stored in a refrigerator at 4°C for almost a year retained a germination level of 84%, compared with its level of 93% immediately after extraction. Pollen which was extracted or stored under conditions different from the above lost a greater amount of viability. There were significant interactions between extraction temperature, moisture content and type of storage: while pollen in less than optimal condition lost viability very rapidly under poor storage modalities, pollen extracted and dried as above was able to tolerate poor storage relatively well. | id |