Uji biopotensi antibodi monoklonal anti pregnant mare serum gonadotropin pada sapi perah

Abstract

The experiment was conducted to determine whether MoAb anti pregnant mare serum gonadotropin (PMSG) (PHB I1 Product) possess neutralizing capacity and to determine an effective neutralizing biopotency against commercial PMSG used in dairy heifer cattle. Twenty five Fries Holland (FH) heifer donors were randomly divided into 5 groups. Group I as a negative control was superovulated with 2000 IU PMSG intramuscularly (i.m.) at 03:OO PM. Donors in group 11, 111, IV, and V received the same treatment as those in group I, but were also administered with MoAb intravenously (i.v.) 30 minutes after PMSG injection. Group I1 as a positive control received 3.3 ml commercial MoAb (Neutra PMSG), whereas groups 111, IV, and V received 0.5 ml; 1.0 ml and 2.0 ml MoAb (PHB I1 Product), respectively. Biopotency test in dairy heifer donors, showed that 2000 IU PMSC used for superovulation was found to be not neutralized by 0.5 ml in group I11 and 1.0 ml of MoAb (PHB I1 Product) in group IV. Superovulation reaction observed were not signifiantly different (M.05) from that observed in group I donors (untreated with MoAb). However when the dose was increased to 2.0 ml, superovulation reaction in treated donors group V were significantly different (P<0.01) from that observed in untreated donors. The result also showed that antibody treatments in group I1 (3.3 ml Neutra PMSC) or group V (2.0 ml PHB I1 Product) could decreased significantly number of functional structure of ovaries and number of collected embryos. In conclusion, based on this finding MoAb (PHB I1 Product) possess neutralizing capacity with the effective neutralizing biopotency to 2000 IU PMSG was estimated as 2.0 ml.