Screening of Proteolytic Enzymes of Sh.eptomyces sp. Local Strain and Their Characterization
Date
2007Author
Yuratmoko, Deri
Mubarik, Nisa Rachmania
Meryandini, Anja
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Protcmse of two Slreptnmyces sp. strain were chosen Cot characterization betaust of the large cltnr zone surroundlng the colomy In nutrient agar media containing 1% (wlv) akim milk. Extracellular proteam from tbt two Isolatcs SLW 8-1 and 451-3 were cbaractertzed following incubation of the isolate in Nutrlent Brotb media containing sklm milk or chicken feather (1%). Tbe optimum tctivlty of the prottnse SLW 8-1 war at pH 9 and 80 'C, wherean that of the ktratinnsc wms at pH 6.5 and 70 'C. Proteast or straln 451-3 showtd its optimum attlvity at pH 7.5 and 50 *C whereas the kerrtiaast was at pH 8.5 mod 80 T.
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