Begomovirus Associated with Pepper Yellow Leaf Curl Disease in West Jva, Indonesia

Abstract

Chili pepper {Capsicum frlltescel1s} showill~ rello" leaf curl from West .J:n'a were analyscd for the presence of a g.::minj.,.-irus. Detection using specific primers for geOlinivirus was conducted following whitefly (Bemisia (abaci Genn.) transmission. A DNA fragment of 1.6 kb W:IS amplified in the polymerase chain reaction using primers which anneal within the replication initiator prolein gene and coal prolein gene. D~A clone of the p(JI~'merase chain reaction product was then sequenced. The slem loop region was found. included the conserved nonanucleolide sequence TAATATTAC present in all geminiviru5. The geminivirus associated with yellow leaf curl disease in peppa showed the highest sc~ucnce identity 93 and 98% with pepper yellow leaf Ctlrllndom'sia \'irm,- from C Dnntlllm and pepper yellow leaf curlilldonesia \'irus from Lycopersicoll escull!ntum, respectively.