Isolasi Dan Identifikasi Imunoglobulin Gama (Igg) Serum Ayam B W S Dan Ayam Ras Dengan Metode Kromatografi Pertukaran Ion Dan Imunokimia')

Abstract

The sady was designed to isolate and identify senun IgG of native and imported chickens, after being immunized with Newcastle Disease Veccine. The isolation method used was the DEAE-Cellulose ion ex-e chromatography using 0.01 M Tris-HCI buffer, at pH 8.0, with linear gradient NaCl from 0.01 M to 0.30 M after salting out with anhydrous NaS04. Identification of IgG characteristics was carried out using the Ouchbrlony QuMe bnmunodifftsion, immuno-electrophorcsis and SDS-PAGE 8% methods. S e m M d o n of native and impmtul chickem using the DEAE-Cellulose chromatography, after salting eat with anhydrous N@@ of 18.14 and 14*4 resulted in four peaks of protein fractions. Fractions 1,2 and 3 showed positive redone with specific rabbit anti chicken IgG (SIGMA) on Ouchterlony double immunodiffusion.