Pengaruh Serum Homolog Dan Heterolog Terhadap Proses Pematangan Dan Pembuahan Oosit Domba Di Dalam Medium Biakan In Vitro

Abstract

The influence of various types of sera i.e. Fetal Calf Serum (FCS), Bovine Post Estrous Serum (BPES) as heterolog sera; and Ewes Fetal Serum (EFS) and Ewes Serzrrn (ES) as homologue sera on in vitro maturation and fertilization of sheep oocytes was investigated s sing -l'iss~ie Culture Medium 1 991-M (TCM 199. Ciibco). Bovinc sera wcre collected I'rom cow at seben days post-estrus, while ewes sera were collected during estrous (ES-HO) and six days post estrous (ES-H6). The result shows that the maturation rate of oocytes cultured in medium supplemented with EFS, ES-HO and ES-H6 at concentrations of 32.9 %, 68.7 %, and 67.6 % respectively is better than those supplemented with FCS and BPES at concentrations of 22.2 % and 28.9 %, respectively. In vitro fertilization rate were significantly higher in medium supplemented with ES-HO and ES-H6 at concentration of 65.4 % and 65.8 % (P < 0.05) than that supplemented with BPES at concentration of 31.7 %. In conclusion, ewes sera could be used in culture medium for promoting both in vitro maturation and fertilization rate of sheep oocytes. Key words: ewes serum, in vitro maturation, in vitr