Ekstraksi Insulin dari Ikan Uji Kemurniannya

Abstract

lnsulin one of hormon prepared from the pancreas of cattle. especially from B cell of langerhans island. Fish insulin associated with gall bladder or bite duct Fullher more fish insulin is normally more slable after the death of the fish, compared with insulin of cattle. lnsulin easy to sparated and prepsred from fish conlinously by DNA recombinand. Insulin is used for the treatment of diabetes, in case lo prepared the insulin who valid lor all of the religions, fish insulin is prepared. The preparation procedure concist of fixing by pieric acid. extraclion and refin. The results 16.5% instrlin in form solution or 10% insul~n in form crislalin. so Tanikama 1965 procedure is better for fish insulin extraction. TLC analysts prod~ce two spol stmm ar rv.th the standar (conlro I R speclrofotomlr c prod~ce am aa ar.2 ;elon component for slanaar and am da. carboks~lal an0 alk I component for Oslt IIP-C analysis produce six amino acid for standar and five amino acid for flsh All of amino acid in side change of fish insulin is similar lo amlno acid of horrnon insulin. Thai the reason of fish insulin have no alergen posibilily.