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      Pengaruh Hidrolisis dan Waktu Ekstraksi terhadap Kandungan Fenolik dan Aktivitas Antioksidan Tanaman Obat Tradisional

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      Date
      2025
      Author
      Hannashery, Kezia Diandra
      Artika, I Made
      Nurcholis, Waras
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      Abstract
      Tanaman temulawak (Curcuma xanthorrhiza), temu hitam (Curcuma aeruginosa), gandarusa (Justicia gendarussa), dan daun ungu (Graptophyllum pictum) merupakan tanaman obat yang memiliki potensi sebagai antioksidan. Penelitian ini bertujuan untuk mgenganalisis efek dari hidrolisis dan perbedaan waktu perebusan terhadap kadar total fenolik dan aktivitas antioksidan. Perlakuan yang dilakukan pada ekstraksi yaitu perebusan 15 menit, perebusan 30 menit dan hidrolisis. Penelitian ini menggunakan metode Folin-Ciocalteu untuk penentuan kadar total fenolik dan metode Ferric Reducing Antioxidant Power (FRAP) untuk mementukan aktivitas antioksidan. Hasil menunjukan kadar total fenolik tertinggi didapatkan dari tanaman daun ungu dengan perlakuan hidrolisis sebesar 43,17 Mg GAE/g. Aktivitas antioksidan pada tanaman gandarusa dengan perebuas 30 menit menunjukan hasil terbesar dengan 99,51 mmol TE/g DW.
       
      Javanese tumeric (Curcuma xanthorrhiza), pink and blue ginger (Curcuma aeruginosa), Gandarusa (Justicia gendarussa), and caricature plant (Graptophyllum pictum) are medicinal plants that have potential as antioxidants. This study aims to analyze the effects of hydrolysis and different boiling times on total phenolic content and antioxidant activity. The treatments carried out in the extraction were boiling for 15 minutes, boiling for 30 minutes and hydrolysis. This study used the Folin-Ciocalteu method to determine total phenolic content and the Ferric Reducing Antioxidant Power (FRAP) method to determine antioxidant activity. The results showed that the highest total phenolic content was obtained from the purple leaf plant with a hydrolysis treatment of 43.17 Mg GAE/g. The antioxidant activity of the gandarusa plant with a 30-minute boil showed the highest results with 99.51 mmol TE/g DW.
       
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      http://repository.ipb.ac.id/handle/123456789/171715
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      • UT - Biochemistry [1465]

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      Copyright © 2020 Library of IPB University
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      Indonesia DSpace Group 
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