Karakterisasi Metabolit Sekunder Ranting Araucaria cunninghamii Menggunakan LC-MS/MS.

Abstract

Araucaria cunninghamii merupakan salah satu spesies dari genus Araucaria yang diketahui mengandung berbagai metabolit sekunder dengan potensi bioaktivitas. Meskipun daun tanaman ini telah banyak diteliti, bagian rantingnya masih jarang dieksplorasi. Penelitian ini bertujuan mengidentifikasi dan mencirikan metabolit sekunder dari ranting A. cunninghamii menggunakan teknik LC-MS. Sampel diekstraksi dengan pelarut metanol, diikuti pemisahan fraksi secara kromatografi, dan analisis profil metabolit menggunakan LC-MS/MS. Fraksi FAn14D menunjukkan keberadaan enam senyawa, yaitu meranzin dan ostol (kumarin), asam fenilpropanoat, dan asam benzoat (asam fenolat), 4',4''',7,7''-tetra- O-metilkupresuflavon (biflavonoid), dan (2E)-3-(3,4-dihidroksifenil) akrilat 9,19- siklolanos-24-en-3-il (triterpenoid lanostanoid). Asam benzoat terdeteksi sebagai senyawa mayor dengan luas 45% pada mode ionisasi negatif. Selain itu, senyawa dioktil adipat juga terdeteksi sebagai artefak yang diduga berasal dari kontaminasi alat selama proses analisis.

Araucaria cunninghamii is one of several species in the Araucaria genus that are known to possess secondary metabolites having bioactive properties. While its leaves have been extensively researched, research into twig-derived compounds remains scarce. The purpose of this work was to evaluate the diversity of secondary metabolites found in A. cunninghamii twigs and characterize their chemical structure using LC-MS spectroscopy. The sample was extracted using methanol as the solvent, followed by chromatographic fractionation and metabolite profiling with LC-MS/MS equipment. The FAn14D fraction from the methanol extract contained six identified compounds, including meranzin and osthol (coumarin group), phenylpropanoic acid and benzoic acid (phenolic acid group), 4',4''',7,7''- tetra-O-methylcupressuflavone (biflavonoid group), and (2E)-3-(3,4-dihydroxy phenyl) acrylate 9,19-cyclolanost-24-en-3-yl (triterpenoid lanostanoid group). The primary constituent was identified as benzoic acid, which had a peak area percentage of 45% in the negative ionization mode. Dioctyl adipate was also discovered, which is thought to be an artifact caused by instrumental contamination during the analytical procedure.