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      Aktivitas Biodegradasi Mikroplastik Oleh Bakteri Simbion Spons Agelas conifera

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      Date
      2025
      Author
      Toding, Jesica Febriola
      Srimariana, Endang Sunarwati
      Ismet, Meutia Samira
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      Abstract
      Mikroplastik merupakan salah satu limbah lautan yang dapat menjadi ancaman bagi ekosistem laut. Memanfaatkan mikroorganisme seperti bakteri dapat menjadi solusi untuk mengurangi permasalahan limbah mikroplastik. Penelitian ini bertujuan untuk mengetahui kemampuan bakteri simbion spons Agelas conifera dalam mendegradasi mikroplastik serta mengidentifikasi jenis bakteri pendegradasi mikroplastik. Metode yang digunakan dalam penelitian ini adalah metode KrocBoral dengan membuat lubang pada media uji dan dilakukan perhitungan bobot akhir mikroplastik. Isolat bakteri kemudian diuji pewarnaan Gram dan uji fisiologi untuk mengindentifikasi jenis bakteri. Hasil penelitian didapatkan bakteri simbion spons Agelas conifera dapat mendegradasi mikroplastik PVC, PE, PET, dan LLDPE hingga sebesar 2,99% selama 20 hari inkubasi pada suhu 30 °C. Hasil uji morfologi makroskopik dan mikroskopik serta uji fisiologi didapatkan 6 genus bakteri simbion spons Agelas conifera yang dapat mendegradasi mikroplastik, yaitu Moraxella, Rhodococcus, Planococcus, Paracoccus, Micrococcus, dan Acinetobacter.
       
      Microplastics are one of the ocean waste materials that can threaten marine ecosystems. Utilizing microorganisms such as bacteria can be a solution to reduce microplastic waste problems. This study aims to determine the ability of symbiotic bacteria from the sponge Agelas conifera to degrade microplastics and to identify the types of microplastic degrading bacteria. The method used in this research was the Kroc-Boral method by creating wells in the test medium, followed by measuring the final weight of the microplastics. Bacterial isolates were then subjected to Gram staining and physiological tests to identify the bacterial types. The results showed that symbiotic bacteria from Agelas conifera could degrade PVC, PE, PET, and LLDPE microplastics by up to 2.99% over a 20-day incubation period at 30 °C. Macroscopic and microscopic morphological tests and physiological test identified six genera of sponge associated bacteria capable of degrading microplastics, Moraxella, Rhodococcus, Planococcus, Paracoccus, Micrococcus, and Acinetobacter.
       
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      http://repository.ipb.ac.id/handle/123456789/169977
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      • UT - Marine Science And Technology [2093]

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