Aplikasi Povidone Iodine pada Tubifex sp. untuk Pencegahan Penyakit Motile Aeromonas Hydrophila (MAS) pada Benih Ikan Mas Koki Ranchu Carassius auratus

Abstract

Ikan mas koki menempati urutan kedua dalam komoditas ikan hias dengan permintaan ekspor tertinggi. Tingginya permintaan ini menyebabkan meningkatnya produksi budidaya. Produksi budidaya ikan mas koki ranchu banyak terkendala penyakit MAS. Problem solving ini bertujuan mengevaluasi pengaplikasian Povidone-Iodine (PVP-I) dalam mencegah penularan A. hydrophila melalui Tubifex sp. pada benih ikan mas koki ranchu. Identifikasi bakteri dilakukan dengan kit API 20E, dilanjutkan uji in vitro dan in vivo. Percobaan ini menggunakan metode RAL dengan 3 perlakuan 4 ulangan. Hasil menunjukkan bahwa dosis PVP-I 50 µL L?¹ selama 15 menit tanpa pembilasan efektif menurunkan bakteri A. hydrophila hingga 0 CFU g?¹ tanpa menyebabkan kematian cacing, serta menghasilkan sintasan benih tertinggi. PVP-I terbukti potensial sebagai disinfektan pakan alami dalam pencegahan penyakit MAS.

Goldfish is the second most exported ornamental fish commodity. This growing demand has driven an increase in aquaculture production. However, ranchu goldfish farming is often challenged by Motile Aeromonas Septicemia (MAS), a disease commonly associated with the use of live feed such as Tubifex sp., which can carry Aeromonas hydrophila. This study aimed to evaluate the application of Povidone-Iodine (PVP-I) in preventing the transmission of A. hydrophila through Tubifex sp. to ranchu fry. Bacterial identification was carried out using the API 20E kit, followed by both in vitro and in vivo tests. The experiment used a Completely Randomized Design (CRD) with 3 treatments and 4 replications. The results showed that immersing Tubifex sp. in 50 µL L?¹ of PVP-I for 15 minutes without rinsing effectively eliminated A. hydrophila until 0 CFU g?¹ without harming the worms, and led to the highest fry survival rate. These findings suggest that PVP-I is a promising and practical disinfectant for natural live feed to help prevent MAS outbreaks in goldfish hatcheries.