Kelimpahan Komunitas Bakteri Heterotrof Terisolasi dari Sedimen Perakaran Mangrove Rhizophora mucronata di Aceh Timur

Abstract

Penelitian ini bertujuan menganalisis kelimpahan dan morfologi bakteri, serta mengidentifikasi jenis bakteri dari sedimen pada perakaran mangrove R. mucronata di ekosistem mangrove rehabilitasi Kuala Langsa dan ekosistem mangrove alami Telaga Tujuh. Metode yang digunakan dalam penelitian yaitu Total Plate Count (TPC) dengan mengisolasi sampel sedimen melaui tahapan pengenceran. Isolat yang berhasil dipurifikasi kemudian diidentifikasi melalui karakterisasi morfologi koloni dan sel, serta uji fisiologis. Hasil penelitian menujukkan wilayah Telaga Tujuh memiliki kelimpahan bakteri lebih tinggi dibandingkan Kuala Langsa. Hasil uji morfologi koloni dan sel, serta uji fisiologis, ditemukan 14 genus bakteri yang terisolasi dari kedua wilayah. Kuala Langsa ditemukan 9 genus bakteri yaitu Staphylococcus, Alcaligenes, Flavobacterium, Marinococcus, Corynebacterium, Acinetobacter, Moraxella, Rhizobacter, Lactococcus. Telaga Tujuh teridentifikasi 7 genus bakteri yaitu Staphylococcus, Marinococcus, Micrococcus, Arthrobacter, Streptococcus, Bacillus, dan Bacteroides.

This study aims to analyze the abundance and morphology of bacteria, as well as identify the type of bacteria from sediments in mangrove roots of R. mucronata in Kuala Langsa rehabilitation mangrove ecosystem and Telaga Tujuh natural mangrove ecosystem. The method used in the study was Total Plate Count (TPC) by isolating sediment samples through the dilution stage. Isolates that were successfully purified were then identified through characterization of colony and cell morphology, and physiological tests. The results showed that the Telaga Tujuh area had a higher bacterial abundance than Kuala Langsa. The results of colony and cell morphology tests, as well as physiological tests, found 14 genus of bacteria isolated from both regions. Kuala Langsa found 9 genus of bacteria namely Staphylococcus, Alcaligenes, Flavobacterium, Marinococcus, Corynebacterium, Acinetobacter, Moraxella, Rhizobacter, Lactocccus. Telaga Tujuh identified 7 genus of bacteria namely Staphylococcus, Marinococcus, Micrococcus, Arthrobacter, Streptococcus, Bacillus, and Bacteroides