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      Deteksi Spesies Moluska dan Chordata yang Berpotensi Invasif Menggunakan DNA Lingkungan di Selat Malaka

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      Date
      2025
      Author
      Ramadhan, Farhan
      Subhan, Beginer
      Lestari, Dea Fauzia
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      Abstract
      Pelabuhan di Selat Malaka menjadi hotspot bagi spesies invasif karena terdapat substrat buatan yang belum terkolonisasi, hal ini memberikan perlindungan dari predator alami. Kehadirannya dapat menurunkan keanekaragaman hayati lokal. Penelitian ini bertujuan deteksi spesies berpotensi invasif dari Filum Moluska dan Chordata melalui pendekatan DNA lingkungan di Pelabuhan Selat Malaka. DNA lingkungan diperoleh dari sampel perairan delapan pelabuhan di Selat Malaka, DNA tersebut diekstraksi dan diamplifikasi menggunakan primer forward mlCOIintF dan primer reverse dgHCO-2198 untuk mengidentifikasi potongan pendek DNA Moluska dan Chordata. Hasil analisis sekuens menggunakan mBRAVE menghasilkan 13,732 Operational Taxonomic Units (OTUs). Total 62 OTUs teridentifikasi spesies asing (49 Moluska dan 13 Chordata) dan dominan di tujuh pelabuhan yang diamati, menunjukkan pelabuhan menjadi tempat utama introduksi spesies asing. Perophora sagamiensis dari kelas Actinopterygii (Chordata), Cnemidocarpa verrucosa dari kelas Ascidiacea (Chordata), dan Crepidula convexa dari kelas Gastropoda (Moluska) menunjukkan potensi invasif yang perlu diwaspadai.
       
      The port in the Malacca Strait is a hotspot for invasive species because there are uncolonized artificial substrates, which provide protection from natural predators. Their presence can reduce local biodiversity. This study aims to detect potentially invasive species from the phyla Mollusca and Chordata through an environmental DNA approach in the Port of Malacca Strait. Environmental DNA was obtained from water samples collected from eight ports in the Malacca Strait. The DNA was extracted and amplified using the forward primer mlCOIintF and the reverse primer dgHCO-2198 to identify short DNA fragments of Mollusca and Chordata. Sequence analysis using mBRAVE yielded 13,732 Operational Taxonomic Units (OTUs). A total of 62 OTUs were identified as alien species (49 Mollusca and 13 Chordata) and were dominant in seven of the observed ports, indicating that ports serve as primary sites for the introduction of alien species. Perophora sagamiensis from the class Actinopterygii (Chordata), Cnemidocarpa verrucosa from the class Ascidiacea (Chordata), and Crepidula convexa from the class Gastropoda (Mollusca) show invasive potential that needs to be monitored.
       
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      http://repository.ipb.ac.id/handle/123456789/165220
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      • UT - Marine Science And Technology [2093]

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      Copyright © 2020 Library of IPB University
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      Indonesia DSpace Group 
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