Rancang Bangun Instrumentasi Pencitraan Mikroskopik Berbasis Raspberry Pi Terintegrasi Lensa Objektif dan Reversed Lens untuk Pengamatan Stomata

Abstract

Penelitian ini berhasil merancang dan membangun instrumen pencitraan mikroskopik berbasis Raspberry Pi yang terintegrasi dengan lensa objektif 40× dan reversed lens untuk pengamatan stomata daun secara real-time. Antarmuka pengguna grafis (GUI) berbasis Python dikembangkan untuk mengontrol kamera dan mengambil gambar secara manual dan berkala. Kinerja instrumen diuji menggunakan linear micrometer ruler, epidermis bawang, daun tomat (Lycopersicum esculentum), dan daun adam eva (Rhoeo discolor). Hasil pengujian pada objek linear micrometer ruler dan epidermis bawang menunjukkan citra yang jelas dengan luas bidang pandang 417,0 µm × 234,6 µm. Stomata pada daun adam eva dapat terlihat dan terdeteksi dengan panjang 81 µm – 84 µm, sedangkan stomata daun tomat tidak teramati akibat trikoma dan ketidakrataan permukaan daun. Upaya pemantauan dinamis pembukaan dan penutupan stomata daun adam eva selama 27 jam terkendala pergeseran sampel dan dehidrasi lokal diduga akibat pencahayaan terus menerus. Rancangan ini menunjukkan potensi mikroskop portabel yang fleksibel untuk studi jaringan tumbuhan.

This study successfully designed and developed a microscopic imaging instrument based on Raspberry Pi, integrated with a 40× objective lens and a reversed lens, for real-time observation of leaf stomata. A Python-based graphical user interface (GUI) was developed to control the camera and capture images manually and at regular intervals. The instrument's performance was tested using a linear micrometer ruler, onion epidermis, tomato leaf (Lycopersicum esculentum), and adam eve leaf (Rhoeo discolor). Test results on the micrometer and onion epidermis showed clear images with a field of view of 417.0 µm × 234.6 µm. Stomata on Rhoeo discolor leaves were clearly visible and measurable, with lengths ranging from 81 µm to 84 µm, while stomata on tomato leaves were not observable due to the presence of trichomes and uneven leaf surfaces. A 27-hour dynamic observation of stomatal opening and closing in Rhoeo discolor was hindered by sample displacement and localized dehydration, presumably caused by continuous illumination. This design demonstrates the potential of a flexible, portable microscope for plant tissue studies.